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12/12/2017 · The single-nucleotide deletion caused formation of a premature stop codon in the open reading frame of bcsmr1OS, resulting in production of a 465-aa truncated protein. The transcription activity of the truncated protein was greatly reduced, compared to that of the 935-aa full-length protein encoded by bcsmr1BS in the BS isolates. more


Draft for internal testing Scientific Committee guidance

6/8/2018 · Based on transmission EM, Dales and Mosbach proposed in 1968 that these spheres are composed of a single open membrane made de novo in the host cytoplasm. This model was heavily criticized by Sodeik and Krijnse-Locker (reviewed in Ref. [18] ) as such a mechanism of membrane biogenesis had no precedent in the cellular and viral worlds. more


Transcriptional networks in plant immunity - Tsuda - 2015

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Protocol for the scientific opinion on the Tolerable Upper

The green fluorescent protein (GFP) and its variants have been widely used in modern biology as reporters that allow a variety of live-cell imaging techniques. So far, GFP has rarely been used in the gray mold fungus Botrytis cinerea because of low fluorescence intensity. The codon usage of B. cinerea genes strongly deviates from that of commonly used GFP-encoding genes and reveals a lower GC more


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6/22/2018 · Introduction. The ATP‐binding cassette (ABC) transporter family is the largest transporter gene family that is present in all living organisms (Jones & George, 2004; Davidson et al., 2008).Most ABC transporter proteins hydrolyze ATP to transport inorganic ions, metals, peptides, steroids, nucleosides, sugars and many other small molecules across extra‐ and intracellular membranes … more


Enzyme Fusions in Biocatalysis: Coupling Reactions by

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Living Colors in the Gray Mold Pathogen Botrytis cinerea

9/12/2014 · Non-covalent and covalent imprinting strategies have been investigated for the synthesis of stigmasterol imprinted polymers. The synthesized molecularly imprinted polymers (MIPs) were then evaluated for their recognition and selectivity towards stigmasterol via static and dynamic batch-binding assays and their performance measured against control non-imprinted polymers (NIPs). more


Dhole - Wikipedia

Even more intriguing is the fact that according to most electron microscopists, including all the original observers of poxvirus formation and many microscopists still working today, the composition of the growing crescents and the final spheres is a single membrane bilayer (Dales and Siminovitch, 1961; Dales, 1963; Patrizi and Middelkamp, 1968; Dales and Mosbach, 1968; Pogo and Dales, 1969 more


Single Mosbach - downloadsteps

A novel process for the removal of impurities from clavulanic acid using a selective adsorption material, in particular a molecularly imprinted polymer. Novel selective adsorption materials suitable for the process, and a process for the preparation of such selective adsorption materials, are also disclosed. more


EP1319086A1 - Process for the preparation of clavulanic

For example, a single blood measure of a persistent substance such as dioxins that has an elimination half‐life of several years could be considered a reliable marker of long‐term exposure and of relevance for most long‐term health outcomes, including chronic disease such as cancer or cardiovascular disease. more


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Translation would then lead to a single polypeptide with multiple enzymes covalently fused. Alternatively, it is possible to create enzyme fusions post‐translationally by using pairs of tags or scaffolds, of which there are several elegant examples, 13 although this type of approach is beyond the scope of this review. more


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If a single study is reported in more than one publication, different publications reporting on the same study (e.g. on different outcomes (endpoints), at different time points) will be identified at this step. The WG may consider using an algorithm to combine the scores in a weighted or unweighted manner: if so, the rationale for the more


Biologically Coupled Gate Field-Effect Transistors Meet in

8/22/2003 · 4. Eva Rosina Stelz bapt. 10 Oct 1751, Guttenbach, Kreis Eberbach, Baden.Married 22 July 1777, Dallau, Kreis Mosbach, Baden. m.Georg Adam Mathes son of Georg Adam Mathew and Anastasiae 5.Georg Stelz b. 1754 d. 30 Jun 1835 at 4:00 p.m., Guttenbach, Kreis Eberbach, Baden.Single never married. Generation III more


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US5597531A US07/397,106 US39710689A US5597531A US 5597531 A US5597531 A US 5597531A US 39710689 A US39710689 A US 39710689A US 5597531 A US5597531 A US 5597531A Authority US United States Prior art keywords magnetic particles recited coating coated Prior art date 1985-10-04 Legal status (The legal status is an assumption and is not a legal conclusion. more


(PDF) Nanostructured Materials: Basic Concept and

The dhole (/ d oʊ l /; Cuon alpinus) is a canid native to Central, South, East, and Southeast Asia.Other English names for the species include Asian wild dog, Asiatic wild dog, Indian wild dog, whistling dog, red dog, and mountain wolf. [page needed] It is genetically close to species within the genus Canis, (Fig. 10) but distinct in several anatomical aspects: its skull is convex rather than more


A comparison of covalent and non-covalent imprinting

11/9/2016 · For single-clone analyses, we re-suspended the cells to a concentration of 2 × 10 6 cells per ml and sorted the GFP − and GFP + cells. The cells were then expanded in … more


US5597531A - Resuspendable coated magnetic particles and

Introduction. Trinucleotide repeat expansions are involved in at least two dozens dramatic neurological and developmental disorders in human , , , , .A large amount of studies were devoted to understanding the mechanisms responsible for large CAG/CTG repeat expansions, using model systems as diverse as bacteria , , yeast , , , drosophila , mice , , , or human cell lines , , . more


Frontiers | Precise Excision of the CAG Tract from the

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Single M%C3%A4nner Mosbach

In perfect-crystal silicon at T 0 K, P(y ) exhibits a single d-function peak at the tetrahedral angle y t 109X478 [86]. manner [32]. However, this is rarely observed except. more


Highly Specific Contractions of a Single CAG/CTG

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Entry and Disassembly of Large DNA Viruses: Electron

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Knowledge Graph Approach to Combustion Chemistry and

My wife and I eat out, a lot. This is, undoubtedly some of the best Italian food we have eaten in Louisville. Not only that it is presented in a friendly, casual manner by servers who are excellent at what they do. On top of this Silvio took the time to come out and speak to us like we were old customers and had been coming there for years. more


Defects in the Ferroxidase That Participates in the

The importance of such multi-scale modeling is more extensively elaborated on by Kraft and Mosbach (Reference Kraft and Mosbach 2010). Figure 2 shows a few entities from the power network scenario in a very simplified manner. The box with the label “Gen1” contains only a single triple “Gen1 is a PowerGenerator.” Meanwhile, a power more


Small RNAs from the plant pathogenic fungus Sclerotinia

These strategies include the silencing of both HTT alleles in a non-allele-selective strategy and the targeting of single-nucleotide polymorphisms (SNPs) linked to repeat expansions. The repeat region itself may be targeted in an allele selective and non-selective manner (Fiszer et al., 2012; Keiser et al., 2016; Esteves et al., 2017). more


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The indicated WRKY transcription factors (blue color) encoded by some of these WRKY genes have also been shown to modulate the expression of other WRKY genes, both in a positive or negative manner, in different tested systems (gray arrows/lines; see Llorca et al., 2014). AtPR1 is a downstream target of AtWRKY6 (Robatzek & Somssich, 2002). Solid more


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7/30/2019 · Plant resistance to several fungal pathogens relies on the recognition of a single fungal effector by a plant resistance protein in a ‘gene‐for‐gene’ manner (Fenton et al., 2009; Jones and Dangl, 2006). This leads to simple segregation between fully susceptible and fully resistant plants in … more


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In this paper, recent works on biologically coupled gate field-effect transistor (bio-FET) sensors are introduced and compared to provide a perspective. Most biological phenomena are closely related to behaviors of ions and biomolecules. This is why biosensing devices for detecting ionic and biomolecular charges contribute to the direct analysis of biological phenomena in a label-free and more


A gapless genome sequence of the fungus Botrytis cinerea

The positive sense single stranded RNA genome of 11 kb has one long open reading frame giving rise to a single polyprotein comprising three structural proteins and seven nonstructural proteins (Rice et al., 1985). Flaviviruses are spherical enveloped viruses with a diameter of approx. 50 nm, and an electron‐dense core of 30 nm. more


Deep-etch EM reveals that the early poxvirus envelope is a

Following earlier incomplete and fragmented versions of a genome sequence for the grey mould Botrytis cinerea, a gapless, near‐finished genome sequence for B. cinerea strain B05.10 is reported. The assembly comprised 18 chromosomes and was confirmed by an optical map and a genetic map based on approximately 75 000 single nucleotide polymorphism (SNP) markers. more


Single M%C3%A4nner Mosbach

The corresponding single nucleotide polymorphisms (SNP) position were derived from the sequencing of ca. 10,000 targeted loci that were part of the BovineSNP50 Illumina Beadchip. After removing duplicated positions (n = 690), genotypes for 9038 SNPs were retained. Note that the resulting data set was very sparsely genotyped. more


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